Mechanical Stimulation of Dermal Fibroblasts at the Association of Dutch Burn Centres
Hi, I'm Caleb Horst for CellScale Biomaterials. Today I am speaking with Marcel Vlig, a senior researcher at the Association of Dutch Burn Centers in Beverwijk, the Netherlands. Marcel is studying the cellular response of dermal fibroblasts to mechanical loading. Thanks Marcel for joining us. I'd like to start just by asking you to tell us a little bit about your research and the goals and what you're hoping to accomplish. - We're trying--we're investigating the mechanical tension, mechanical loading to dermal fibroblasts because when people suffer from burn wounds, wounds want to contract.
And in the clinic I use pins to reverse that contraction. And we want to know what the effect is of this mechanical loading to cells. And how are you using the MechanoCulture for the testing that you're doing, and how does it help you understand the response of the cells? - We use the MechanoCulture to set up different stretching regimes, dynamic stretching or static stretching or a combination of these two to see which one is has the most positive effect on dermal fibroblasts. And the biggest advantage of the MechanoCulture is that we can use 3D collagen scaffolds to do our experiments with. - Can you walk us through a typical test, Marcel, and just explain to us how you load the cells onto your device and how you run the test? -Yeah, I will use a collagen scaffold, which we sterilized previously to do our experiment, and then we attach it onto our snowflake using a sponge...push the pins through our collagen scaffold, just like this. We use tweezers to place the collagen scaffold. When it's in the device we can seed our cells onto the collagen scaffold and we wait for two hours so they can adhere to the scaffold. Then we fill up the chamber with medium, wait for 24 hours so they can expand and grow, and then we start our protocol which is 24 hours of stretch or static stretch, and after that collect RNA from our cells.
- And what stage is your research at, Marcel? Have you been able to show how the cells respond to mechanical loading using the MechanoCulture? - We've now established the number of cells we need to seed in our collagen scaffold to obtain proper results and we also say that if we apply a stretch regime on our cells, they do respond to this stress compared to the control cells. So we do have a working system and now we have to apply different kinds of stretch regimes to see which one is more beneficial. - Thanks Marcel, and thank you for watching this video.
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